Můžete přidat SET
klauzule na konec dotazu, např.
LOAD DATA LOCAL INFILE 'C:/mydata.csv'
INSERT INTO TABLE myTable
FIELDS TERMINATED BY ','
OPTIONALLY ENCLOSED BY '"'
LINES TERMINATED BY '\r\n'
IGNORE 1 LINES
(Column1,Column2,Column3,Column4)
SET datetime = NOW();